RESUMO Objetivos: descrever o perfil alimentar e analisar o Sistema Sensório Motor Oral em neonatos prematuros e de baixo peso que participaram ou não do Método Canguru. Métodos: trata-se de um estudo observacional não-intervencionista, quantitativo, realizado com 60 neonatos prematuros e de baixo peso. Foi utilizado um formulário específico para descrição do perfil alimentar. Nas avaliações do sistema sensório motor oral dos neonatos utilizou-se, o protocolo de Castro(1). Resultados: os neonatos prematuros e de baixo peso avaliados formaram dois grupos: 30 participantes do Método Canguru (G1) e 30 não participantes deste método (G2). Apresentaram Idade Gestacional de 25 a 36 semanas, sendo 34 do sexo feminino e 26 do sexo masculino; 8 neonatos se alimentavam por sonda, 46 por aleitamento materno exclusivo, 4 recebiam aleitamento materno e copinho; e 1 apenas pelo copinho. No Grupo1, 9(30%) neonatos apresentaram todos os reflexos orais dentro do esperado. Já no Grupo 2, 12 (40%) apresentou todos os reflexos presentes. Conclusão: este estudo revela que os neonatos que passaram pelo Método Canguru não obtiveram diferença estatisticamente significante em relação ao perfil alimentar e ao sistema sensório motor oral, quando comparados aos neonatos que não passaram por esse método.

ABSTRACT Purposes: to describe the feeding profile and analyze the Oral Sensory Motor System of preterm low birth weight who participated or not the Kangaroo method. Methods: this is a non-interventional, observational quantitative study with 60 preterm low birth weight. A specific form was used to describe the feeding profile. Evaluations of Oral Sensory Motor System were made by the protocol of Castro(1). Results: preterm and low birth weight babies evaluated formed two groups: 30 participants of the Kangaroo Method (G1) and 30 non-participants of this method (G2). They presented Gestational Age 25-36 weeks, which were 34 female and 26 male; 8 children were fed by nasogastric tube, 46 by exclusive breastfeeding, 4 fed through the cup and 1 just by the cup. In the Grupo1,9 (30%) infants showed oral reflexes as expected. In the Group 2, 12 (40%) showed all reflexes. Conclusion: this study shows that babies who went through the kangaroo method do not revealed statistically significant difference in relation to the feeding profile and oral sensory motor system, when compared to babies who did not went through this method.

This study aimed to characterize the resistance of 31 passion fruit (Passiflora sp.) genotypes to Fusarium oxysporum f. sp. passiflorae (FOP). About 20 seedlings of each genotype were inoculated by immersing the roots in a suspension of 10(6) macroconidia mL-1 for five minutes and then transplanting them into pots containing a mixture of soil and sterile substrate. They were evaluated daily for 120 days for the occurrence of wilt symptoms and death. These data were used to calculate the area under the disease progress curve, for survival analysis and grouping. The mortality rate ranged from 0 to 100% and the AUDPC ranged from 0 for genotypes without symptoms of the disease to 6,650.63 for the hybrid HFOP-01. Genotypes were classified into four subgroups: resistant, moderately resistant, susceptible and highly susceptible. The resistance was genotype dependent, but no significant variation was found between the purple and the yellow P. edulis.

Fusarium oxysporum f. sp. passiflorae W.L. Gordon (FOP) is one of the most important fungal pathogens of passion fruits. Understanding molecular variation of isolates from different areas is of utmost importance. Molecular fingerprinting on 14 isolates of FOP were conducted using AFLP molecular markers (Amplified Fragment Length Polymorphism), and their genetic variability were estimated. Twenty-five AFLP primer combinations were selected for amplification of FOP isolates and one for Fusarium oxysporum f. sp. cubense W.C. Snyder & H.N. Hansen (FOC), resulting in 99% polymorphic fragments, with an average of 40 fragments per primer combination. Specific fingerprints could be generated for most of the isolates evaluated; we observed a high power of discrimination of the AFLP primer combinations, with the presence/absence of up to 26 specific fragments per isolate. Thus, specific fingerprinting was obtained for 10 of the 15 isolates analyzed. The values of the polymorphic information content, the index and the resolving power of the markers showed wide variation and reflected the high informative contents of the primers used in the characterization of the FOP isolates. The FOP isolates were divided into four groups, irrespective of their geographic origins, with the allocation of 5, 7, 1 and 1 FOP isolates into Groups II, III, IV and V, respectively. A wide genetic diversity was observed in FOP isolates, which should be taken into consideration when implementing strategies for the improvement of passion fruit in the search for cultivars with multiple resistance to different isolates.

Em virtude da baixa variabilidade genética relatada nos plantios comerciais de mamoeiro (Carica papaya L.), o objetivo deste trabalho foi analisar a divergência genética de 32 genótipos incluindo cultivares, variedades locais, linhagens e germoplasma melhorado, utilizando a técnica de AFLP (Amplified Fragment Length Polymorphism). A matriz de distâncias genéticas foi obtida com a distância de Nei e Li, e o agrupamento foi realizado utilizando o método UPGMA (unweighted pair-method with arithmetic mean). Utilizaram-se 11 combinações de iniciadores com corte das enzimas EcoRI / MseI. Foram geradas 383 bandas polimórficas, com média de 34,8 por combinação de iniciadores. O agrupamento dos dados permitiu a formação de cinco grupos, sendo a cultivar Sunrise e a linhagem CMF-L30-08 os genótipos mais relacionados, e CMF041 (germoplasma melhorado) e CMF233 (variedade local), os mais dissimilares. As cultivares de mamoeiro mais cultivadas no Brasil, bem como quatro linhagens e três acessos de germoplasma melhorado foram agrupadas, porém não no mesmo ramo. Mesmo sendo oriunda de mutação e seleção dentro de Sunrise, a cultivar Golden ainda possui considerável variabilidade genética, uma vez que a distância genética entre estas cultivares foi de 0,329. Variabilidade adicional foi observada nas linhagens do programa de melhoramento.

Due to the low genetic variability reported in the commercial plantations of papaya (Carica papaya L.), the objective of this study was analyze the genetic diversity of 32 genotypes including cultivars, landraces, inbred lines, and improved germplasm using the AFLP technique (Amplified Fragment Length Polymorphism). The genetic distance matrix was obtained using the Nei and Li genetic distance and clustering was performed using the unweighted pair-method with arithmetic mean (UPGMA). Using 11 combinations of EcoRI/MseI primers, 383 polymorphic bands were obtained. On average, 34.8 polymorphic bands were obtained per primer combination. Five clusters were formed. The traditional cultivar 'Sunrise' and the inbred line CMF-L30-08 were the closest genotypes, and the improved germplasm (CMF041) and landrace (CMF233) the most distant. The main papaya cultivars commercially grown in Brazil, as well as four inbred lines and three improved germplasm, were clustered together, however, were not grouped in the same branch. The genetic distance between the Sunrise and Golden cultivars was 0.329, and even arising from mutation and selection within the Sunrise variety, the Golden stores considerable genetic variability. Additional variability was observed in the inbred lines derived from papaya breeding program at Embrapa Cassava and Fruits.